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KMID : 0368420160590020093
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Journal of Plant Biology
2016 Volume.59 No. 2 p.93 ~ p.104
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Modern quantitative analytical tools and biosensors for functional studies of auxin
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Seo Hye-Su
Kriechbaumer Verena
Park Woong-June
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Abstract
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Auxin is one of the most important plant hormones as it diversely regulates growth and development. Because the action of auxin is often correlated with its local distribution and flux, quantitative analysis and monitoring of auxin is indispensable to understanding plant development. Great efforts have been made to detect, visualize, quantify and monitor auxin in order to understand its physiological roles in planta. Initial trials to measure quantitative effects of auxin were bioassays. Chromatographic techniques were then introduced and their applications were expanded when combined with sensitive detection methods. Modern quantitative analysis depends on four major steps: extraction, pretreatment, resolution (separation) and signal detection. GC, HPLC or UPLC combined with tandem mass spectrometry currently are the strongest tools to simultaneously identify and quantify auxin and auxin related substances. In spite of its extreme selectivity and sensitivity, mass spectrometry-based quantification is inconvenient to map the spatial distribution of auxin. On the other hand, quantitative imaging by immunohistochemistry, electrochemical- or bio-sensor is very useful to reveal local auxin distribution which is important for plant developmental regulation. Currently the ‘DII-VENUS biosensor’ was made available. This biosensor is not influenced by the signal transduction processes of auxin. We review useful traditional methods of studying auxin and also focus on recent advances in quantitative analytical techniques and monitoring systems based on biosensors.
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KEYWORD
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Auxin, Biosensor, Imaging, Mass spectrometry, Profiling, Quantification
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